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C17orf74 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C17orf74 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C17orf74 antibodies →
$504.00each
Catalog Number: LS-G104989-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C17orf74 (NM_175734), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C17orf74
Origin Species
Human
NCBI GenBank
NM_175734
SwissProt
Q0P670

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MENQLWHNTV RCCNQYQESP HDAEDILLLL LGLIVLVNIG INVATMMWHG LQNALDKMID
61WATQKNEIQA SESPPSGPPD KAQDVHIHCI LDPVQVKMSR PTQYSSFSCH HFSNHHSSSL
121LRCVRRRRRR HRRCRRRCCN HQQRPQNYRQ IPHSHSVFRN PHRSQKMSQL HRVPFFDQED
181PDSYLEEEDN LPFPYPKYPR RGWGGFYQRA GLPSNVGLWG HQGGILASLP PPSLYLSPEL
241RCMPKRVEAR SELRLQSYGR HGSQSRLWGN VEAEQWASSP PPPHRLPPNP SWVPVGHSPY
301PSVGWMLYDS WDQRRRGTEG FERPPASVSR NARPEAQGCR EHHSPQSHQQ SLLGHAYGQS
361HRSPHPSTEP LGYSSQDPRE VRRRAADWAE ALPAWRPLTT SASLTVLDEA SHQRTPAPSS
421VLVPHSSQPW PKVQAADPAP PPTMFVPLSR NPGGNANYQV YDSLELKRQV QKSRARSSSL
481PPASTSTLRP SLHRSQTEKL N
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

501 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.