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C17orf70 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C17orf70 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C17orf70 antibodies →
$725.00each
Catalog Number: LS-G109551-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FP100(FAAP100) (NM_025161), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C17orf70 | FAAP100
Origin Species
Human
NCBI GenBank
NM_025161
SwissProt
Q0VG06

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQLFEQPCPG EDPRPGGQIG EVELSSYTPP AGVPGKPAAP HFLPVLCSVS PSGSRVPHDL
61LGGSGGFTLE DALFGLLFGA DATLLQSPVV LCGLPDGQLC CVILKALVTS RSAPGDPNAL
121VKILHHLEEP VIFIGALKTE PQAAEAAENF LPDEDVHCDC LVAFGHHGRM LAIKASWDES
181GKLVPELREY CLPGPVLCAA CGGGGRVYHS TPSDLCVVDL SRGSTPLGPE QPEEGPGGLP
241PMLCPASLNI CSVVSLSASP RTHEGGTKLL ALSAKGRLMT CSLDLDSEMP GPARMTTESA
301GQKIKELLSG IGNISERVSF LKKAVDQRNK ALTSLNEAMN VSCALLSSGT GPRPISCTTS
361TTWSRLQTQD VLMATCVLEN SSSFSLDQGW TLCIQVLTSS CALDLDSACS AITYTIPVDQ
421LGPGARREVT LPLGPGENGG LDLPVTVSCT LFYSLREVVG GALAPSDSED PFLDECPSDV
481LPEQEGVCLP LSRHTVDMLQ CLCFPGLAPP HTRAPSPLGP TRDPVATFLE TCREPGSQPA
541GPASLRAEYL PPSVASIKVS AELLRAALKD GHSGVPLCCA TLQWLLAENA AVDVVRARAL
601SSIQGVAPDG ANVHLIVREV AMTDLCPAGP IQAVEIQVES SSLADICRAH HAVVGRMQTM
661VTEQAAQGSS APDLRVQYLR QIHANHETLL REVQTLRDRL CTEDEASSCA TAQRLLQVYR
721QLRHPSLILL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

730 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.