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C17orf53 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C17orf53 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C17orf53 antibodies →
$725.00each
Catalog Number: LS-G110097-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C17orf53 (NM_024032), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C17orf53
Origin Species
Human
NCBI GenBank
NM_024032
SwissProt
Q8N3J3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MACSLQKLFA VEEEFEDEDF LSAVEDAENR FTGSLPVNAG RLRPVSSRPQ ETVQAQSSRL
61LLLHPTAPSE ALGLPDLDLC LPASSTPSAD SRPSCIGAAP LRPVSTSSSW IGNQRRVTVT
121EVLRETARPQ SSALHPLLTF ESQQQQVGGF EGPEQDEFDK VLASMELEEP GMELECGVSS
181EAIPILPAQQ REGSVLAKKA RVVDLSGSCQ KGPVPAIHKA GIMSAQDESL DPVIQCRTPR
241PPLRPGAVGH LPVPTALTVP TQQLHWEVCP QRSPVQALQP LQAARGTIQS SPQNRFPCQP
301FQSPSSWLSG KAHLPRPRTP NSSCSTPSRT SSGLFPRIPL QPQAPVSSIG SPVGTPKGPQ
361GALQTPIVTN HLVQLVTAAS RTPQQPTHPS TRAKTRRFPG PAGILPHQQS GRSLEDIMVS
421APQTPTHGAL AKFQTEIVAS SQASVEEDFG RGPWLTMKST LGLDERDPSC FLCTYSIVMV
481LRKQAALKQL PRNKVPNMAV MIKSLTRSTM DASVVFKDPT GEMQGTVHRL LLETCQNELK
541PGSVLLLKQI GVFSPSLRNH YLNVTPNNLV HIYSPDSGDG SFLKPSQPFP KDSGSFQHDV
601AAKPEEGFRT AQNLEAEASP EEELPEADDL DGLLSELPED FFCGTSS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

647 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.