LSBio
C16orf96 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C16orf96 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C16orf96 antibodies →
$725.00each
Catalog Number: LS-G126475-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C16orf96 (NM_001145011), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001145011

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSFSLTFTEL ANIAIPQCGV LNFKALHLLL HGILEHIHMA ELKKVLSGDE DFLQTSQVVI
61MPREGDAQPI LNPMKRLSNV FDHVVSRLDK LENQLALLQD LPSTAQLLEA SQGTARPVQD
121LWHLIKLRKM VEGHDEVMAK SMQTLQDLLT DLHALQVTIT ALRKEVDMLK NMLDKVHPER
181MDIFAEDFKI QNWKMVALQR EVASLQNKFK TIPKTEDMVL WSGLHDAMFT SEIGSSPLDL
241WQSVEQLPEA ALAQTTKYLE ATRAIQVSEP VQNPQLLQTV WHYEVPELLP EGSSAQAVSL
301SRAQEPAQPP ALTPESAPGC TTEFAPGPAP GTEPVPGLEL GLELEPVPAL GPVPGPSVTP
361GSLPAPWPVL GPVPAPGAQP PPLGDWPALP RRWPLPQGWP RVGSWPLWDL GVLRPTQPQP
421SRAPPPATEF GSLWPRPLQP YQSRQGEALQ LAAVQVKGEE NDVPSLRGLR ERARKDGAPK
481DRTRKDGVPK DRGGKDVDPK DRAHKDDVPK DRGGKDVDPK DRAHKDDVPK DRGGKDGDPK
541DRVGKDGAPK EAQPKAPQSA LHRLKTTAAI AAAAAAAYAA ATSSAAQAAK VAAKFVKDAP
601ATKMAAIATD TAAAGPLGVF ADVLGAGPSR GATESQILGD DSEIYEILSP SYSAASIGPD
661PALSQAMVAT KQAMSPEDKK RAVKYSMSHI AQIPVKHDSL KEEFAQLSCN LNQRLSYLAN
721MGGPSSLGTT VDILQKKIGS LQKSRLKEEE LERIWGNQIE MMKDRYITLD KAVENLQIRM
781DEFKTLQAQI KRLEMNKVNK STMEEELREK ADRSALAGKA SRVDLETVAL ELNEMIQGIL
841FKVTIHEDSW KKAMEELSKD VNTKLVHSDL DPLKKEMEEV WKIVRKLLIE GLRLDPDSAA
901GFRRKLFKRV KCISCDRPVE MMTGPQLITI RKAHLLSRLR PASANSCEYL QRQQMREQQW
961LQLQDLGIQE DCQQDWGDGP QNATSLKCKS CNLLTLYPYG DPHVIDYDSA EVDILGVDGI
1021LYKGRVNSQR GAQPLAVAKE LAAVKAPSPP SQSLYDRVHS SALFGAICPP LCPRSSACSA
1081ASGPHLTMPA RPPSLPPLLL LPPLIPSLRD PQQAPGSTRL SRAPHIESRV GRKPPEEPAN
1141P
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1141 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.