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C16orf71 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C16orf71 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107084-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C16orf71 (NM_139170), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C16orf71
Origin Species
Human
SwissProt
Q8IYS4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASNDKGMAP SLGSPWASRM GPWDAILKAV KDQLPSLDSD SPLSDYGEEE LFIFQRNQTS
61LIPDLSEELA EDPADGDKSR AWVAAAEESL PEPVLVPAEL ATEPGCRQNT RTKDASSQEG
121RDPGRPFESS GEVSALLGMA EEPPRWLEGD LGSLSFNTKG SQGPPWDPQA EATLSCHEGD
181PKAEPLSTAS QESVNRRALR QERRKMIETD ILQKVTRDAC GPTSSDKGGV KEAPCHAAES
241APRSKMPLVE PPEGPPVLSL QQLEAWDLDD ILQSLAGQED NQGNRAPGTV WWAADHRQVQ
301DCMVPSAHNR LMEQLALLCT TQSKASACAR KVPADTPQDT KEADSGSRCA SRKRGSQAGP
361GPQLAQGMRL NAESPTIFID LRQMELPDHL SPESSSHSSS DSEEEEEEEM AALGDAEGAS
421PSSLGLRTCT GKSQLLQQLR AFQKGTAQPE LPASKGPAGG RAQALEDTAG SRTGRKQHMK
481LCAKGQSAQA RLPRGRPRAL GDVPEPGAAR EALMPPLEQL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

520 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.