Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
C16orf70 / LIN10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101944-100
Catalog Number: LS-G101944
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C16orf70 (NM_025187), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Lin-10 homolog | LIN10 | LIN-10 | C16orf6 | C16orf70 | UPF0183 protein C16orf70
- Origin Species
- Human
- NCBI GenBank
- NM_025187
- SwissProt
- Q9BSU1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLDLEVVPER SLGNEQWEFT LGMPLAQAVA ILQKHCRIIK NVQVLYSEQS PLSHDLILNL61TQDGIKLMFD AFNQRLKVIE VCDLTKVKLK YCGVHFNSQA IAPTIEQIDQ SFGATHPGVY121NSAEQLFHLN FRGLSFSFQL DSWTEAPKYE PNFAHGLASL QIPHGATVKR MYIYSGNSLQ181DTKAPMMPLS CFLGNVYAES VDVLRDGTGP AGLRLRLLAA GCGPGLLADA KMRVFERSVY241FGDSCQDVLS MLGSPHKVFY KSEDKMKIHS PSPHKQVPSK CNDYFFNYFT LGVDILFDAN301THKVKKFVLH TNYPGHYNFN IYHRCEFKIP LAIKKENADG QTETCTTYSK WDNIQELLGH361PVEKPVVLHR SSSPNNTNPF GSTFCFGLQR MIFEVMQNNH IASVTLYGPP RPGSHLRTAE421LPTRTRPLEQ KLISEEDLAA NDILDYKDDD DKV
453 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.