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C16orf58 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C16orf58 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110270-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C16orf58 (NM_022744), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C16orf58 | UPF0420 protein C16orf58
Origin Species
Human
NCBI GenBank
NM_022744

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADDAGLETP LCSEQFGSGE ARGCRAAADG SLQWEVGGWR WWGLSRAFTV KPEGRDAGEV
61GASGAPSPPL SGLQAVFLPQ GFPDSVSPDY LPYQLWDSVQ AFASSLSGSL ATQAVLLGIG
121VGNAKATVSA ATATWLVKDS TGMLGRIVFA WWKGSKLDCN AKQWRLFADI LNDVAMFLEI
181MAPVCPICFT MTVSTSNLAK CIVSVAGGAT RAALTVHQAR RNNMADVSAK DSSQETLVNL
241AGLLVSLLML PLVSGCPGFS LGCFFFLTAL HIYANYRAVR ALVMETLNEG RLRLVLKHYL
301QRGEVLDPTA ANRMEPLWTG FWPAPSLSLG VPLHRLVSSV FELQQLVEGH QESYLLCWDQ
361SQNQVQVVLN QKAGPKTILR AATHGLMLGA LQGDGPLPAE LEELRNRVRA GPKKESWVVV
421KETHEVLDML FPKFLKGLQD AGWKTEKHQL EVDEWRATWL LSPEKKVL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

468 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.