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C15orf52 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C15orf52 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C15orf52 antibodies →
$725.00each
Catalog Number: LS-G102787-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C15orf52 (NM_207380), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C15orf52
Origin Species
Human
SwissProt
Q6ZUT6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MISCAEQRSR QGEAGRGPAP VAPAFLPLWL PRGCSGILSV PAVAMHSAGT PRAESPMSRQ
61EKDAELDRRI VALRKKNQAL LRRYQEIQED RRQAEQGGMA VTTPALLQPD GLTVTISQVP
121GEKRVVSRNW ARGTCGPRVT NEMLEDEDAE DHGGTFCLGE LVELAVTMEN KAEGKRIVSE
181KPTRARNQGI EGSPGGRVTR SPPTQVAISS DSARKGSWEP WSRPVGEPPE AGWDYAQWKQ
241EREQIDLARL ARHRDAQGDW RRPWDLDKAK STLQDCSQLR GEGPARAGSR RGPRSHQKLQ
301PPPLLPDGKG RGGQASRPSV APATGSKARG KERLTGRARR WDMKEDKEEL EGQEGSQSTR
361ETPSEEEQAQ KQSGMEQGRL GSAPAASPAL ASPEGPKGES VASTASSVPC SPQEPDLAPL
421DLSLGGAGIP GPRESGCVLG LRPGAQESPV SWPEGSKQQP LGWSNHQAEL EVQTCPEPQR
481GAGLPEPGED RSGKSGAQQG LAPRSRPTRG GSQRSRGTAG VRRRTGRPGP AGRC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

534 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.