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C15orf39 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C15orf39 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C15orf39 antibodies →
$504.00each
Catalog Number: LS-G113180-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C15orf39 (NM_015492), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C15orf39 | DKFZP434H132
Origin Species
Human
NCBI GenBank
NM_015492
SwissProt
Q6ZRI6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
110.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEKRPLRTL GPVMYGKLPR LETDSGLEHS LPHSVGNQDP CTYKGSYFSC PMAGTPKAES
61EQLASWTPYP PLYSTGMAGP PLQADNLLTN CLFYRSPAEG PEKMQDSSPV ELLPFSPQAH
121SYPGPPLAAP KPVYRNPLCY GLSTCLGEGA VKRPLDVDWT LATGPLLPSA DPPCSLAPAP
181SKGQTLDGTF LRGVPAEGSS KDSSGSFSPC QPFLEKYQTI HSTGFLASRY TGPYPRNSKQ
241AMSEGPSSPW TQLAQPLGPP CQDTGPTHYP PPHHPPPHPP QALPCPPACR HPEKQGSYSP
301ALPLQPLGGH KGTGYQAGGL GSPYLRQQAA QAPYIPPLGL DAYPYPSAPL PAPSPGLKLE
361PPLTPRCPLD FAPQTLSFPY ARDDLSLYGA SPGLGGTPPS QNNVRAVPQP GAFQRACQPL
421PASQPCSEPV RPAQEAEEKT WLPSCRKEKL QPRLSEHSGP PIVIRDSPVP CTPPALPPCA
481RECQSLPQKE DARPPSSPPM PVIDNVFSLA PYRDYLDVPA PEATTEPDSA TAEPDSAPAT
541SEGQDKGCRG TLPAQEGPSG SKPLRGSLKE EVALDLSVRK PTAEASPVKA SRSVEHAKPT
601AAMDVPDVGN MVSDLPGLKK IDTEAPGLPG VPVTTDAMPR TNFHSSVAFM FRKFKILRPA
661PLPAAVVPST PTSAPAPTQP APTPTSGPIG LRILAQQPLS VTCFSLALPS PPAVAVASPA
721PAPAPSPAPA RAQAPASARD PAPAPAPVAG PAPASTSAPG DSLEQHFTGL HASLCDAISG
781SVAHSPPEKL REWLETAGPW GQAAWQDCQG VQGLLAKLLS QLQRFDRTHR CPFPHVVRAG
841AIFVPIHLVK ERLFPRLPPA SVDHVLQEHR VELRPTTLSE ERALRELALP GCTSRMLKLL
901ALRQLPDIYP DLLGLQWRDC VRRQLGDFDT EAGAVSSSEP TVARDEPESL ALAQKSPAPK
961VRKPGRKPPT PGPEKAEAAA GEESCGASPT PATSASPPGP TLKARFRSLL ETAWLNGLAL
1021PTWGHKSSRP DQPSPCPQLL DSQSHHL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1047 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.