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C14orf43 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C14orf43 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119425-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C14orf43(ELMSAN1) (NM_001043318), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14_5541 | C14orf117 | C14orf43 | ELMSAN1 | LSR68
Origin Species
Human
SwissProt
Q6PJG2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
114.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNLQAQPKAQ NKRKRCLFGG QEPAPKEQPP PLQPPQQSIR VKEEQYLGHE GPGGAVSTSQ
61PVELPPPSSL ALLNSVVYGP ERTSAAMLSQ QVASVKWPNS VMAPGRGPER GGGGGVSDSS
121WQQQPGQPPP HSTWNCHSLS LYSATKGSPH PGVGVPTYYN HPEALKREKA GGPQLDRYVR
181PMMPQKVQLE VGRPQAPLNS FHAAKKPPNQ SLPLQPFQLA FGHQVNRQVF RQGPPPPNPV
241AAFPPQKQQQ QQQPQQQQQQ QQAALPQMPL FENFYSMPQQ PSQQPQDFGL QPAGPLGQSH
301LAHHSMAPYP FPPNPDMNPE LRKALLQDSA PQPALPQVQI PFPRRSRRLS KEGILPPSAL
361DGAGTQPGQE ATGNLFLHHW PLQQPPPGSL GQPHPEALGF PLELRESQLL PDGERLAPNG
421REREAPAMGS EEGMRAVSTG DCGQVLRGGV IQSTRRRRRA SQEANLLTLA QKAVELASLQ
481NAKDGSGSEE KRKSVLASTT KCGVEFSEPS LATKRAREDS GMVPLIIPVS VPVRTVDPTE
541AAQAGGLDED GKGPEQNPAE HKPSVIVTRR RSTRIPGTDA QAQAEDMNVK LEGEPSVRKP
601KQRPRPEPLI IPTKAGTFIA PPVYSNITPY QSHLRSPVRL ADHPSERSFE LPPYTPPPIL
661SPVREGSGLY FNAIISTSTI PAPPPITPKS AHRTLLRTNS AEVTPPVLSV MGEATPVSIE
721PRINVGSRFQ AEIPLMRDRA LAAADPHKAD LVWQPWEDLE SSREKQRQVE DLLTAACSSI
781FPGAGTNQEL ALHCLHESRG DILETLNKLL LKKPLRPHNH PLATYHYTGS DQWKMAERKL
841FNKGIAIYKK DFFLVQKLIQ TKTVAQCVEF YYTYKKQVKI GRNGTLTFGD VDTSDEKSAQ
901EEVEVDIKTS QKFPRVPLPR RESPSEERLE PKREVKEPRK EGEEEVPEIQ EKEEQEEGRE
961RSRRAAAVKA TQTLQANESA SDILILRSHE SNAPGSAGGQ ASEKPREGTG KSRRALPFSE
1021KKKKTETFSK TQNQENTFPC KKCGR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1045 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.