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C14orf39 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C14orf39 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C14orf39 antibodies →
$725.00each
Catalog Number: LS-G105109-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIX6OS1(C14orf39) (NM_174978), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_174978

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68 kda
Protein Type
Lysate
Amino Acid Sequence
1MNDSLFVSLD RLLLEFVFQY EQDISTKEEM IQRINKCCED IKENKVTICR IHETINATDE
61EIDHYCKHSE EIKDNCRNWK PTCDVFRKHE DYMQDQFTVY QGTVEKDKEM YHDYICQYKE
121VLKQYQLKYS ETPFSREYYE KKREHEEIQS RVLACTEQLK MNETIFMKFR VPAPFPSLTK
181WTLNIVNLRC ETQDILKHAS NLTKSSSELK KEVDEMEIEI NYLNQQISRH NETKALSETL
241EEKNKNTENR KELKERIFGK DEHVLTLNKT QSSQLFLPYE SQKLVRPIKM HSSEPRVADI
301KEESSVKQSK LANIDFRQKE NDTQIFNDSA VDNHSKCSHI TTITSSQKFM QVRLLTPQKQ
361SNSNQWSEKG DKDAEYGDKG TVRQVRESKC TSQAIYTEHF GKSIENDSDE VEERAENFPR
421TSEIPIFLGT PKAVKAPESL EKIKFPKTPP FEINRNRNAV PEVQTEKESP GLSFLMSYTS
481RSPGLNLFDS SVFDTEISSD QFNEHYSARN LNPLSSEQEI GNLFEKPEGE DGFTFSFPSD
541TSTHTFGAGK DDFSFPFSFG QGQNSIPSSS LKGFSSSSQN TTQFTFF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

587 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.