Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
C14orf131 / ZNF839 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111806-100
Catalog Number: LS-G111806
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF839 (NM_018335), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Zinc finger protein 839 | C14orf131 | ZNF839
- Origin Species
- Human
- NCBI GenBank
- NM_018335
- SwissProt
- A8K0R7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 99.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADAEPEAGG GSEDGGGGGG PAPPGQSGSV ARVAPLGPEQ LRQVLEQVTK AQPPPPPPPF61VLRDAARRLR DAAQQAALQR GRGTEPPRLP RLLPPQQLEA ICVKVTSGET KGQERPMLLP121TTIQPQTARK SQLPRGNSCL VGLHIASPQL LRVQPLVRTE PQSCFLSDLC QPPAQGFVQR181PLPALQVVPA KRVPAPKAPD EQGSMLTPLS ASDPLAVTSL SSSSAHPFIS NLHTRHTEKL241KKSLKVKTRS GRVSRPPKYK AKDYKFIKTE DLADGHLSDS DDYSELCVEE DEDQRERHAL301FDLSSCSLRP KSFKCQTCEK SYIGKGGLAR HFKLNPGHGQ LDPEMVLSEK ASGSTLRGCT361EERTLSLTSL GLSMPADPCE GGARSCLVTE SARGGLQNGQ SVDVEETLPS EPENGALLRS421ERYQGPRRRA CSETLAESRT AVLQQRRAAQ LPGGPAAAGE QRASPSKARL KEFLQQCDRE481DLVELALPQL AQVVTVYEFL LMKVEKDHLA KPFFPAIYKE FEELHKMVKK MCQDYLSSSG541LCSQETLEIN NDKVAESLGI TEFLRKKEIH PDNLGPKHLS RDMDGEQLEG ASSEKREREA601AEEGLASVKR PRREALSNDT TESLAANSRG REKPRPLHAL AAGFSPPVNV TVSPRSEESH661TTTVSGGNGS VFQAGPQLQA LANLEARRGS IGAALSSRDV SGLPVYAQSG EPRRLTQAQV721AAFPGENALE HSSDQDTWDS LRSPGFCSPL SSGGGAESLP PGGPGHAEAG HLGKVCDFHL781NHQQPSPTSV LPTEVAAPPL EKILSVDSVA VDCAYRTVPK PGPQPGPHGS LLTEGCLRSL841SGDLNRFPCG MEVHSGQREL ESVVAVGEAM AFEISNGSHE LLSQGQKQIF IQTSDGLILS901PPGTIVSQEE DIVTVTDAEG RACGWARTRTRPLEQKLISEEDLAANDILDYKDDDDKV
927 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.