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C14orf118 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C14orf118 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GPATCH2L antibodies →
$725.00each
Catalog Number: LS-G112115-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C14orf118(GPATCH2L) (NM_017926), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf118 | GPATCH2L
Origin Species
Human
NCBI GenBank
NM_017926
SwissProt
Q9NWQ4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDELVHDLAS ALEQTSEQNK LGELWEEMAL SPRQQRRQLR KRRGRKRRSD FTHLAEHTCC
61YSEASESSLD EATKDCREVA PVTNFSDSDD TMVAKRHPAL NAIVKSKQHS WHESDSFTEN
121APCRPLRRRR KVKRVTSEVA ASLQQKLKVS DWSYERGCRF KSAKKQRLSR WKENTPWTSS
181GHGLCESAEN RTFLSKTGRK ERMECETDEQ KQGSDENMSE CETSSVCSSS DTGLFTNDEG
241RQGDDEQSDW FYEGECVPGF TVPNLLPKWA PDHCSEVERM DSGLDKFSDS TFLLPSRPAQ
301RGYHTRLNRL PGAAARCLRK GRRRLVGKET SINTLGTERI SHIISDPRQK EKNKALASDF
361PHISACAHEF NPLSPLYSLD VLADASHRRC SPAHCSARQA NVHWGPPCSR DIKRKRKPVA
421TASLSSPSAV HMDAVEPTTP ASQAPKSPSS EWLVRTSAAE KATDATTATF FKMPQEKSPG
481YS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

482 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.