Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
C13orf8 / ZNF828 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128786-100
Catalog Number: LS-G128786
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C13orf8(CHAMP1) (NM_001164145), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C13orf8 | ZNF828 | CHAMP | CHAMP1 | KIAA1802 | Zinc finger protein 828
- Origin Species
- Human
- NCBI GenBank
- NM_032436
- SwissProt
- Q96JM3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAFQELRKP SARLECDHCS FRGTDYENVQ IHMGTIHPEF CDEMDAGGLG KMIFYQKSAK61LFHCHKCFFT SKMYSNVYYH ITSKHASPDK WNDKPKNQLN KETDPVKSPP LPEHQKIPCN121SAEPKSIPAL SMETQKLGSV LSPESPKPTP LTPLEPQKPG SVVSPELQTP LPSPEPSKPA181SVSSPEPPKS VPVCESQKLA PVPSPEPQKP APVSPESVKA TLSNPKPQKQ SHFPETLGPP241SASSPESPVL AASPEPWGPS PAASPESRKS ARTTSPEPRK PSPSESPEPW KPFPAVSPEP301RRPAPAVSPG SWKPGPPGSP RPWKSNPSAS SGPWKPAKPA PSVSPGPWKP IPSVSPGPWK361PTPSVSSASW KSSSVSPSSW KSPPASPESW KSGPPELRKT APTLSPEHWK AVPPVSPELR421KPGPPLSPEI RSPAGSPELR KPSGSPDLWK LSPDQRKTSP ASLDFPESQK SSRGGSPDLW481KSSFFIEPQK PVFPETRKPG PSGPSESPKA ASDIWKPVLS IDTEPRKPAL FPEPAKTAPP541ASPEARKRAL FPEPRKHALF PELPKSALFS ESQKAVELGD ELQIDAIDDQ KCDILVQEEL601LASPKKLLED TLFPSSKKLK KDNQESSDAE LSSSEYIKTD LDAMDIKGQE SSSDQEQVDV661ESIDFSKENK MDMTSPEQSR NVLQFTEEKE AFISEEEIAK YMKRGKGKYY CKICCCRAMK721KGAVLHHLVN KHNVHSPYKC TICGKAFLLE SLLKNHVAAH GQSLLKCPRC NFESNFPRGF781KKHLTHCQSR HNEEANKKLM EALEPPLEEQ QITRTRPLEQKLISEEDLAANDILDYKDDDDKV
812 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.