LSBio
C12orf26 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C12orf26 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all METTL25 antibodies →
$504.00each
Catalog Number: LS-G108950-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C12orf26(METTL25) (NM_032230), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
METTL25 | Methyltransferase like 25 | C12orf26
Origin Species
Human
NCBI GenBank
NM_032230
SwissProt
Q8N6Q8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68 kda
Protein Type
Lysate
Amino Acid Sequence
1MAASCPLPVT PDLPTLRAKL QGLLQFLRDA LSISNAHTVD FYTESVWEEL VDLPPETVLA
61ALRKSASETE ALPSETRPLV EAEWEAGMTD FPKIFCETSQ KLVSVEAFAL AAKYYSVQNL
121GICTPFEQLL VALRGNQNQR IGENQKAVEF MNMKKSHEVQ AMSELISSIA DYYGIKQVID
181LGSGKGYLSS FLSLKYGLKV YGIDSSNTNT HGAEERNRKL KKHWKLCHAQ SRLDVNGLAL
241KMAKERKVKN KVKNKADTEE VFNNSPTNQE KMPTSAILPD FSGSVISNIR NQMETLHSQP
301HQEENLCFEN SFSLINLLPI NAVEPTSSQQ IPNRETSEAN KERRKMTSKS SESNIYSPLT
361SFITADSELH DIIKDLEDCL MVGLHTCGDL APNTLRIFTS NSEIKGVCSV GCCYHLLSEE
421FENQHKERTQ EKWGFPMCHY LKEERWCCGR NARMSACLAL ERVAAGQGLP TESLFYRAVL
481QDIIKDCYGI TKCDRHVGKI YSKCSSFLDY VRRSLKKLGL DESKLPEKII MNYYEKYKPR
541MNELEAFNML KVVLAPCIET LILLDRLCYL KEQEDIAWSA LVKLFDPVKS PRCYAVIALK
601KQQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

603 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.