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C11orf80 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C11orf80 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C11orf80 antibodies →
$504.00each
Catalog Number: LS-G109858-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C11orf80 (NM_024650), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C11orf80 | FLJ22531
Origin Species
Human
NCBI GenBank
NM_024650
SwissProt
Q8N6T0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MVASAGSLFG GMVLKKFLKE IQSILPGISA KLTWTSEEGS YSQDMTGVTP FQMIFEVDEK
61PRTLMTDCLV IKHFLRKIIM VHPKVRFHFS VKVNGILSTE IFGVENEPTL NLGNGIALLV
121DSQHYVRPNF GTIESHCSRI HPVLGHPVML FIPEDVAGMD LLGELILTPA AALCPSPKVS
181SNQLNRISSV SIFLYGPLGL PLILSTWEQP MTTFFKDTSS LVDWKKYHLC MIPNLDLNLD
241RDLVLPDVSY QVESSEEDQS QTMDPQGQTL LLFLFVDFHS AFPVQQMEIW GVYTLLTTHL
301NAILVESHSV VQGSIQFTVD KVLEQHHQAA KAQQKLQASL SVAVNSIMSI LTGSTRSSFR
361KMCLQTLQAA DTQEFRTKLH KVFREITQHQ FLHHCSCEVK QQLTLEKKDS AQGTEDAPDN
421SSLELLADTS GQAENKRLKR GSPRIEEMRA LRSARAPSPS EAAPRRPEAT AAPLTPRGRE
481HREAHGRALA PGRASLGSRL EDVLWLQEVS NLSEWLSPSP GP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

522 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.