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C11orf63 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C11orf63 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C11orf63 antibodies →
$504.00each
Catalog Number: LS-G109751-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C11orf63 (NM_024806), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C11orf63
Origin Species
Human
NCBI GenBank
NM_024806
SwissProt
Q6NUN7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSKRKLIPKL SIQSPVLHTN LNVQSTHPPL KKEDLHRISK DSLESDSESL TQEIMCHSEF
61DDRIRGNGME PDSLDEEESP RWGSLHEMEE EASGKAAQMA REQNHHTWDQ GANNRQQPIE
121DKYSDLRYDP NWKSKKEEGQ LLSVEALPES TDSSLENLPL APLYPSQETS MELSGGKGEQ
181KESPQSAASL LGSEFLSPNY EHGARRSKPF SELSDSDLEE KSSSLSPYVK SSSSHNEVFL
241PGSRGPRRRK SKQHFVEKNK LTLGLPTPKT DSYLQLHNKK RGESHPEQIS YPVRVTDKTS
301IQNAKEMENA AIDPEDKWHQ RAQQLKNYQE HWSQYESTKS SNVPRGQPSD MVNDHQPSRR
361PAKLKIRKQC KHQNGLKSST TEEVTASQGN QNNPPRQQQN QNKPLDTSTK PESIVIMHAS
421NNDVQASRAL RSHNLKETSN TFAPPKQAFD KVLSKNSTGC DSGLNVNKER GHKDQEEKRF
481SYQQLHTLSD MDLNNLNELS KRHVLLSQKG SQFVYHINTH GSTKNKKQLK QPYTETKYRN
541LEMLWKFHSS SDSQTVRASP DSWLTQIMEQ HQQALVQLTD VQPSEGALSS VTLPPILSRV
601ESESQLSSER SQRNQVKISR SNSEGYLFQL EKGKKHKKRS SSKNTKLKGY QKRDVKLGGL
661GPDFESIRDK TQKLIQQKEY AKQVKEYNMK TLSILSKPQT EKTQKKSAIP RQKALEYAKT
721IPKPKPSNLT HQASKEQKNP TYAGKEESLP EISLLEILQN RHEREKQAVA AFKVLHIV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

778 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.