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C10orf90 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C10orf90 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G122556-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C10orf90 (NM_001004298), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C10orf90 | Centrosomal protein C10orf90 | FATS | RP11-422P15.2 | BA422P15.2
Origin Species
Human
NCBI GenBank
NM_001004298
SwissProt
Q96M02

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MLKLSGEGLR DSYHSRRDQI ALKNLQSDVT EAKSDFTKET LASQNTKMIS SIVISQMIDE
61NKSRENRASL PLPCAIAQSR AHHAKQSLAN RSGVNIHRAF ALLPGRLGIP APSDERGPEA
121ELPPKEERPC GGPRRGFASI TITARRVGPP ARALVWGTAG DSLCPKCRAE DTLFQAPPAL
181ANGAHPGRHQ RSFACTEFSR NSSVVRLKVP EAHTGLCERR KYWVTHADDK ETSFSPDTPL
241SGKSPLVFSS CVHLRVSQQC PDSIYYVDKS LSVPIEPPQI ASPKMHRSVL SLNLNCSSHR
301LTADGVDGLV NREPISEALK QELLEGDQDL VGQRWNPGLQ ESHLKETPSL RRVHLGTGAC
361PWSGSFPLEN TELANVGANQ VTVRKGEKDH TTHCHASDHA NQLSIHIPGW SYRAVHTKVF
421SGSSKRQQGE VCMTVSAPPV EQKPTRHFLP IGDSSPSDDC LSRDLSEPTE RRHQSFLKPR
481ILFPGFLCPL QDVCASLQED NGVQIESKFP KGDYTCCDLV VKIKECKKSE DPTTPEPSPA
541APSPAPRDGA GSPGLSEDCS ESQQTPARSL TLQEALEVRK PQFISRSQER LKKLEHMVQQ
601RKAQRKEDLR QKQSLLPIRT SKKQFTIPHP LSDNLFKPKE RCISEKEMHM RSKRIYDNLP
661EVKKKKEEQR KRVILQSNRL RAEVFKKQLL DQLLQRNAV
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

699 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.