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BTNL9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BTNL9 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106184-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BTNL9 (NM_152547), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BTN3 | BTNL9 | Butyrophilin 3 | Butyrophilin-like 9 | VDLS1900 | Butyrophilin-like protein 9
Origin Species
Human
NCBI GenBank
AA931528

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVDLSVSPDS LKPVSLTSSL VFLMHLLLLQ PGEPSSEVKV LGPEYPILAL VGEEVEFPCH
61LWPQLDAQQM EIRWFRSQTF NVVHLYQEQQ ELPGRQMPAF RNRTKLVKDD IAYGSVVLQL
121HSIIPSDKGT YGCRFHSDNF SGEALWELEV AGLGSDPHLS LEGFKEGGIQ LRLRSSGWYP
181KPKVQWRDHQ GQCLPPEFEA IVWDAQDLFS LETSVVVRAG ALSNVSVSIQ NLLLSQKKEL
241VVQIADVFVP GASAWKSAFV ATLPLLLVLA ALALGVLRKQ RRSREKLRKQ AEKRQEKLTA
301ELEKLQTELD WRRAEGQAEW RAAQKYAVDV TLDPASAHPS LEVSEDGKSV SSRGAPPGPA
361PGHPQRFSEQ TCALSLERFS AGRHYWEVHV GRRSRWFLGA CLAAVPRAGP ARLSPAAGYW
421VLGLWNGCEY FVLAPHRVAL TLRVPPRRLG VFLDYEAGEL SFFNVSDGSH IFTFHDTFSG
481ALCAYFRPRA HDGGEHPDPL TICPLPVRGT GVPEENDSDT WLQPYEPADP ALDWW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

535 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.