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BTN1A1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BTN1A1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G99537-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BTN1A1 (NM_001732), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BTN1A1 | BTN | BT | Butyrophilin
NCBI GenBank
NM_001732
SwissProt
Q13410

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVFPSSGLP RCLLTLILLQ LPKLDSAPFD VIGPPEPILA VVGEDAELPC RLSPNASAEH
61LELRWFRKKV SPAVLVHRDG REQEAEQMPE YRGRATLVQD GIAKGRVALR IRGVRVSDDG
121EYTCFFREDG SYEEALVHLK VAALGSDPHI SMQVQENGEI CLECTSVGWY PEPQVQWRTS
181KGEKFPSTSE SRNPDEEGLF TVAASVIIRD TSTKNVSCYI QNLLLGQEKK VEISIPASSL
241PRLTPWIVAV AVILMVLGLL TIGSIFFTWR LYNERPRERR NEFSSKERLL EELKWKKATL
301HAVDVTLDPD TAHPHLFLYE DSKSVRLEDS RQKLPEKTER FDSWPCVLGR ETFTSGRHYW
361EVEVGDRTDW AIGVCRENVM KKGFDPMTPE NGFWAVELYG NGYWALTPLR TPLPLAGPPR
421RVGIFLDYES GDISFYNMND GSDIYTFSNV TFSGPLRPFF CLWSSGKKPL TICPIADGPE
481RVTVIANAQD LSKEIPLSPM GEESAPRDAD TLHSKLIPTQ PSQGAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

526 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.