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BTBD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BTBD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111940-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BTBD7(BTBD7) (NM_018167), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BTB (POZ) domain containing 7 | BTBD7 | FUP1 | KIAA1525
Origin Species
Human
NCBI GenBank
AK001510
SwissProt
Q9P203

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
45.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGANASNYPH SCSPRVGGNS QAQQTFIGTS SYSQQGYGCE SKLYSLDHGH EKPQDKKKRT
61SGLATLKKKF IKRRKSNRSA DHAKQMRELL SGWDVRDVNA LVEEYEGTSA LKELSLQASL
121ARPEARTLQK DMADLYEYKY CTDVDLIFQE TCFPVHRAIL AARCPFFKTL LSSSPEYGAE
181IIMDINTAGI DMPMFSALLH YLYTGEFGME DSRFQNVDIL VQLSEEFGTP NSLDVDMRGL
241FDYMCYYDVV LSFSSDSELV EAFGGNQNCL DEELKAHKAV ISARSPFFRN LLQRRIRTGE
301EITDRTLRTP TRIILDESII PKKYATVILH CMYTDVVDLS VLHCSPSVGS LSEVQALVAG
361KPNMTRAEEA MELYHIALFL EFNMLAQEET TVIRPACAAE LSNSCLLPQS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

410 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.