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BTBD16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BTBD16 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all BTBD16 antibodies →
$725.00each
Catalog Number: LS-G106946-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BTBD16 (NM_144587), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BTB (POZ) domain containing 16 | BTBD16 | Em:AC061711.1 | C10orf87
Origin Species
Human
SwissProt
Q32M84

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIMSNTQHKA RLERRVTGST NRWRLPKQPF SGDLLSLSQM CKALSIDFEE ALRNPDRLCI
61SQIQKFFFEN FKNKDIQSGE ADVILECLGF KWELHQPQLF QSETLAKLYL KALAQGTTHP
121LRELEELLRA QSPKKTKEKS PAKRIIISLK INDPLVTKVA FATALKNLYM SEVEINLEDL
181LGVLASAHIL QFSGLFQRCV DVMIARLKPS TIKKFYEAGC KYKEEQLTTG CEKWLEMNLV
241PLGGTQIHLH KIPQDLLHKV LKSPRLFTFS EFHLLKTMLL WVFLQLNYKI QAIPTYETVM
301TFFKSFPENC CFLDRDIGRS LRPLFLCLRL HGITKGKDLE VLRHLNFFPE SWLDQVTVNH
361YHALENGGDM VHLKDLNTQA VRFGLLFNQE NTTYSKTIAL YGFFFKIKGL KHDTTSYSFY
421MQRIKHTDLE SPSAVYEHNH VSLRAARLVK YEIRAEALVD GKWQEFRTNQ IKQKFGLTTS
481SCKSHTLKIQ TVGIPIYVSF AFIFPAS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

507 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.