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BTBD11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BTBD11 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all BTBD11 antibodies →
$725.00each
Catalog Number: LS-G121273-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BTBD11 (NM_001017523), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001017523

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRKLRPKDSR EPAPGSPVRS GCLQRLSHYS GIQRPFLVLP PLMEWIRVAV AHAGHRRSFS
61MDSDDVRQAA RLLLPGVDCE PRQLRADDCF CASRKLDAVA IEAKFKQDLG FRMLNCGRTD
121LVKQAVSLLG PDGINTMSEQ GMTPLMYACV RGDEAMVQML LDAGADLNVE VVSTPHKYPS
181VHPETRHWTA LTFAVLHGHI PVVQLLLDAG AKVEGSVEHG EENYSETPLQ LAAAVGNFEL
241VSLLLERGAD PLIGTMYRNG ISTTPQGDMN SFSQAAAHGH RNVFRKLLAQ PEKEKSDILS
301LEEILAEGTD LAETAPPPLC ASRNSKAKLR ALREAMYHSA EHGYVDVTID IRSIGVPWTL
361HTWLESLRIA FQQHRRPLIQ CLLKEFKTIQ EEEYTEELVT QGLPLMFEIL KASKNEVISQ
421QLCVIFTHCY GPYPIPKLTE IKRKQTSRLD PHFLNNKEMS DVTFLVEGRP FYAHKVLLFT
481ASPRFKALLS SKPTNDGTCI EIGYVKYSIF QLVMQYLYYG GPESLLIKNN EIMELLSAAK
541FFQLEALQRH CEIICAKSIN TDNCVDIYNH AKFLGVTELS AYCEGYFLKN MMVLIENEAF
601KQLLYDKNGE GTGQDVLQDL QRTLAIRIQS IHLSSSKGSV V
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

641 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.