Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BTBD10 / GMRP-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108913-100
Catalog Number: LS-G108913
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BTBD10 (NM_032320), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BTBD10 | GMRP-1 | BTB (POZ) domain containing 10 | GMRP1 | KSARCOSIN
- Origin Species
- Human
- NCBI GenBank
- NM_032320
- SwissProt
- Q9BSF8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGRPHPYDG NSSDPENWDR KLHSRPRKLY KHSSTSSRIA KGGVDHTKMS LHGASGGHER61SRDRRRSSDR SRDSSHERTE SQLTPCIRNV TSPTRQHHVE REKDHSSSRP SSPRPQKASP121NGSISSAGNS SRNSSQSSSD GSCKTAGEMV FVYENAKEGA RNIRTSERVT LIVDNTRFVV181DPSIFTAQPN TMLGRMFGSG REHNFTRPNE KGEYEVAEGI GSTVFRAILD YYKTGIIRCP241DGISIPELRE ACDYLCISFE YSTIKCRDLS ALMHELSNDG ARRQFEFYLE EMILPLMVAS301AQSGERECHI VVLTDDDVVD WDEEYPPQMG EEYSQIIYST KLYRFFKYIE NRDVAKSVLK361ERGLKKIRLG IEGYPTYKEK VKKRPGGRPE VIYNYVQRPF IRMSWEKEEG KSRHVDFQCV421KSKSITNLAA AAADIPQDQL VVMHPTPQVD ELDILPIHPP SGNSDLDPDA QNPMLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
475 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.