Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BRUNOL5 / CELF5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110557-100
Catalog Number: LS-G110557
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BRUNOL5(CELF5) (NM_021938), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Brunol5 | CELF-5 | Bruno-like protein 5 | CELF5 | CUG-BP and ETR-3 like factor 5 | BRUNOL-5 | RNA-binding protein BRUNOL-5
- Origin Species
- Human
- NCBI GenBank
- NM_021938
- SwissProt
- Q8N6W0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MARLTESEAR RQQQQLLQPR PSPVGSSGPE PPGGQPDGMK DLDAIKLFVG QIPRHLDEKD61LKPLFEQFGR IYELTVLKDP YTGMHKGCAF LTYCARDSAI KAQTALHEQK TLPGMARPIQ121VKPADSESRG GRDRKLFVGM LNKQQSEEDV LRLFQPFGVI DECTVLRGPD GSSKGCAFVK181FSSHTEAQAA IHALHGSQTM PGASSSLVVK FADTDKERTL RRMQQMVGQL GILTPSLTLP241FSPYSAYAQA LMQQQTTVLS TSGSYLSPGV AFSPCHIQQI GAVSLNGLPA TPIAPASGLH301SPPLLGTTAV PGLVAPITNG FAGVVPFPGG HPALETVYAN GLVPYPAQSP TVAETLHPAF361SGVQQYTAMY PTAAITPIAH SVPQPPPLLQ QQQREGPEGC NLFIYHLPQE FGDTELTQMF421LPFGNIISSK VFMDRATNQS KCFGFVSFDN PASAQAAIQA MNGFQIGMKR LKVQLKRPKD481PGHPYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
485 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.