Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BRUNOL4 / CELF4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G123697-100
Catalog Number: LS-G123697
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BRUNOL4(CELF4) (NM_001025087), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BRUNOL-4 | Brunol5 | CELF-4 | CELF4 | Bruno-like protein 4 | CUG-BP and ETR-3 like factor 4 | LYST-interacting protein LIP9 | RNA-binding protein BRUNOL-4 | BRUNOL4 | RNA-binding protein BRUNOL4
- Origin Species
- Human
- NCBI GenBank
- NM_020180
- SwissProt
- Q9BZC1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MYIKMATLAN GQADNASLST NGLGSSPGSA GHMNGLSHSP GNPSTIPMKD HDAIKLFIGQ61IPRNLDEKDL KPLFEEFGKI YELTVLKDRF TGMHKGCAFL TYCERESALK AQSALHEQKT121LPGMNRPIQV KPADSESRGG SSCLRQPPSQ DRKLFVGMLN KQQSEDDVRR LFEAFGNIEE181CTILRGPDGN SKGCAFVKYS SHAEAQAAIN ALHGSQTMPG ASSSLVVKFA DTDKERTMRR241MQQMAGQMGM FNPMAIPFGA YGAYAQAMQQ QAALMASVAQ GGYLNPMAAF AAAQMQQMAA301LNMNGLAAAP MTPTSGGSTP PGITAPAVPS IPSPIGVNGF TGLPPQANGQ PAAEAVFANG361IHPYPAQSPT AADPLQQAYA GVQQYAGPAA YPAAYGQISQ AFPQPPPMIP QQQREGPEGC421NLFIYHLPQE FGDAELMQMF LPFGFVSFDN PASAQTAIQA MNGFQIGMKR LKVQLKRPKD481ANRPYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
485 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.