Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BRINP2 / FAM5B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110741-100
Catalog Number: LS-G110741
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BRINP2 (NM_021165), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BRINP2 | DBCCR1L2 | FAM5B | DBCCR1-like protein 2 | DBCCR1-like2 | KIAA1747 | Protein FAM5B | RP5-1026E2.1
- Origin Species
- Human
- NCBI GenBank
- NM_021165
- SwissProt
- Q9C0B6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRWQCGTRFR GLRPAVAPWT ALLALGLPGW VLAVSATAAA VVPEQHASVA GQHPLDWLLT61DRGPFHRAQE YADFMERYRQ GFTTRYRIYR EFARWKVNNL ALERKDFFSL PLPLAPEFIR121NIRLLGRRPN LQQVTENLIK KYGTHFLLSA TLGGEESLTI FVDKQKLGRK TETTGGASII181GGSGNSTAVS LETLHQLAAS YFIDRESTLR RLHHIQIATG AIKVTETRTG PLGCSNYDNL241DSVSSVLVQS PENKVQLLGL QVLLPEYLRE RFVAAALSYI TCSSEGELVC KENDCWCKCS301PTFPECNCPD ADIQAMEDSL LQIQDSWATH NRQFEESEEF QALLKRLPDD RFLNSTAISQ361FWAMDTSLQH RYQQLGAGLK VLFKKTHRIL RRLFNLCKRC HRQPRFRLPK ERSLSYWWNR421IQSLLYCGES TFPGTFLEQS HSCTCPYDQS SCQGPIPCAL GEGPACAHCA PDNSTRCGSC481NPGYVLAQGL CRPEVAESLE NFLGLETDLQ DLELKYLLQK QDSRIEVHSI FISNDMRLGS541WFDPSWRKRM LLTLKSNKYK PGLVHVMLAL SLQICLTKNS TLEPVMAIYV NPFGGSHSES601WFMPVNEGSF PDWERTNVDA AAQCQNWTIT LGNRWKTFFE TVHVYLRSRI KSLDDSSNET661IYYEPLEMTD PSKNLGYMKI NTLQVFGYSL PFDPDAIRDL ILQLDYPYTQ GSQDSALLQL721IELRDRVNQL SPPGKVRLDL FSCLLRHRLK LANNEVGRIQ SSLRAFNSKL PNPVEYETGK781LCSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
783 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.