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BRD9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BRD9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all BRD9 antibodies →
$504.00each
Catalog Number: LS-G121651-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BRD9 (NM_001009877), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Bromodomain containing 9 | BRD9 | PRO9856 | Sarcoma antigen NY-SAR-29 | LAVS3040
Origin Species
Human
NCBI GenBank
NM_023924

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.25 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKGYQSLVFN FFFLKLSAEN ESTPIQQLLE HFLRQLQRKD PHGFFAFPVT DAIAPGYSMI
61IKHPMDFGTM KDKIVANEYK SVTEFKADFK LMCDNAMTYN RPDTVYYKLA KKILHAGFKM
121MSKQAALLGN EDTAVEEPVP EVVPVQVETA KKSKKPSREV ISCMFEPEGN ACSLTDSTAE
181EHVLALVEHA ADEARDRINR FLPGGKMGYL KRNGDGSLLY SVVNTAEPDA DEEETHPVDL
241SSLSSKLLPG FTTLGFKDER RNKVTFLSSA TTALSMQNNS VFGDLKSDEM ELLYSAYGDE
301TGVQCALSLQ EFVKDAGSYS KKVVDDLLDQ ITGGDHSRTL FQLKQRRNVP MKPPDEAKVG
361DTLGDSSSSV LEFMSMKSYP DVSVDISMLS SLGKVKKELD PDDSHLNLDE TTKLLQDLHE
421AQAERGGSRP SSNLSSLSNA SERDQHHLGS PSRLSVGEQP DVTHDPYEFL QSPEPAASAK
481T
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

481 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.