Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BRAT1 / BAAT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106023-100
Catalog Number: LS-G106023
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BAAT1(BRAT1) (NM_152743), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BAAT1 | BRAT1 | C7orf27 | RMFSL
- Origin Species
- Human
- SwissProt
- Q6PJG6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDPECAQLLP ALCAVLVDPG QPVADDTCLE KLLDWFKTVT EGESSVVLLQ EHPCLVELLS61HVLKVQDLSS GVLSFSLRLA GTFAAQENCF QYLQQGELLP GLFGEPGPLG RATWAVPTVR121SGWIQGLRSL AQHPSALRFL ADHGAVDTIF SLQGDSSLFV ASAASQLLVH VLALSMRGGA181EGQPCLPGGD WPACAQKIMD HVEESLCSAA TPKVTQALNV LTTTFGRCQS PWTEALWVRL241SPRVACLLER DPIPAAHSFV DLLLCVARSP VFSSSDGSLW ETVARALSCL GPTHMGPLAL301GILKLEHCPQ ALRTQAFQVL LQPLACVLKA TVQAPGPPGL LDGTADDATT VDTLLASKSS361CAGLLCRTLA HLEELQPLPQ RPSPWPQASL LGATVTVLRL CDGSAAPASS VGGHLCGTLA421GCVRVQRAAL DFLGTLSQGT GPQELVTQAL AVLLECLESP GSSPTVLKKA FQATLRWLLS481SPKTPGCSDL GPLIPQFLRE LFPVLQKRLC HPCWEVRDSA LEFLTQLSRH WGGQADFRCA541LLASEVPQLA LQLLQDPESY VRASAVTAMG QLSSQGLHAP TSPEHAEARQ SLFLELLHIL601SVDSEGFPRR AVMQVFTEWL RDGHADAAQD TEQFVATVLQ AASRDLDWEV RAQGLELALV661FLGQTLGPPR THCPYAVALP EVAPAQPLTE ALRALCHVGL FDFAFCALFD CDRPVAQKSC721DLLLFLRDKI ASYSSLREAR GSPNTASAEA TLPRWRAGEQ AQPPGDQEPE AVLAMLRSLD781LEGLRSTLAE SSDHVEKSPQ SLLQDMLATG GFLQGDEADC YTRTRPLEQKLISEEDLAANDILDYKDDDDKV
821 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.