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BRAP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BRAP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G100908-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BRAP (NM_006768), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BRAP2 | BRAP | IMP | Galectin-2-binding protein | RING finger protein 52 | RNF52 | BRCA1 associated protein | BRCA1-associated protein
Origin Species
Human
NCBI GenBank
NM_006768
SwissProt
Q7Z569

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSVSLVVIRL ELAEHSPVPA GFGFSAAAGE MSDEEIKKTT LASAVACLEG KSPGEKVAII
61HQHLGRREMT DVIIETMKSN PDELKTTVEE RKSSEASPTA QRSKDHSKEC INAAPDSPSK
121QLPDQISFFS GNPSVEIVHG IMHLYKTNKM TSLKEDVRRS AMLCILTVPA AMTSHDLMKF
181VAPFNEVIEQ MKIIRDSTPN QYMVLIKFRA QADADSFYMT CNGRQFNSIE DDVCQLVYVE
241RAEVLKSEDG ASLPVMDLTE LPKCTVCLER MDESVNGILT TLCNHSFHSQ CLQRWDDTTC
301PVCRYCQTPE PVEENKCFEC GVQENLWICL ICGHIGCGRY VSRHAYKHFE ETQHTYAMQL
361TNHRVWDYAG DNYVHRLVAS KTDGKIVQYE CEGDTCQEEK IDALQLEYSY LLTSQLESQR
421IYWENKIVRI EKDTAEEINN MKTKFKETIE KCDNLEHKLN DLLKEKQSVE RKCTQLNTKV
481AKLTNELKEE QEMNKCLRAN QVLLQNKLKE EERVLKETCD QKDLQITEIQ EQLRDVMFYL
541ETQQKINHLP AETRQEIQEG QINIAMASAS SPASSGGSGK LPSRKGRSKR GK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

592 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.