Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BPIFC / BPIL2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105152-100
Catalog Number: LS-G105152
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BPIFC (NM_174932), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BPI fold containing family C | BPIFC | BPIL2 | BPI-like 2 | DJ149A16.7
- Origin Species
- Human
- SwissProt
- Q8NFQ6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MCTKTIPVLW GCFLLWNLYV SSSQTIYPGI KARITQRALD YGVQAGMKMI EQMLKEKKLP61DLSGSESLEF LKVDYVNYNF SNIKISAFSF PNTSLAFVPG VGIKALTNHG TANISTDWGF121ESPLFQDTGG ADLFLSGVYF TGIIILTRND FGHPTLKLQD CYAQLSHAHV SFSGELSVLY181NSFAEPMEKP ILKNLNEMLC PIIASEVKAL NANLSTLEVL TKIDNYTLLD YSLISSPEIT241ENYLDLNLKG VFYPLENLTD PPFSPVPFVL PERSNSMLYI GIAEYFFKSA SFAHFTAGVF301NVTLSTEEIS NHFVQNSQGL GNVLSRIAEI YILSQPFMVR IMATEPPIIN LQPGNFTLDI361PASIMMLTQP KNSTVETIVS MDFVASTSVG LVILGQRLVC SLSLNRFRLA LPESNRSNIE421VLRFENILSS ILHFGVLPLA NAKLQQGFPL SNPHKFLFVN SDIEVLEGFL LISTDLKYET481SSKQQPSFHV WEGLNLISRQ WRGKSAPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
507 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.