Bovine TMPRSS15 / Enterokinase Protein (Recombinant 6His, C-terminus)

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Expression System	Other
Format:	Lyophilized

$279.00each

Catalog Number: LS-G27030-10

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Overview

Description: Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys(DDDDK(up)). Enterokinase will not work if the recognition site is followed by a proline. rbEKhas the highest activity than EK of other species and is used widely in biochemical applications. rbEK with 6 x His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system. Recombinant Bovine Enterokinase (His-tagged) (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids, 6 x His at C-terminus. A fully biologically active molecule, rbEK has a molecular mass of 40 kDa and is obtained by chromatographic techniques.
Category: Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names: Enteropeptidase | Enterokinase | ENTK | PRSS7 | Serine protease 7 | TMPRSS15
Origin Species: Bovine
NCBI GenBank: NM_002772|NP_002763.2
SwissProt: P98073

Protein

Expression System: Other
Fusion Tag: Hexahistidine Tag
Molecular Weight: 40 kDa (SDS-PAGE)
Protein Type: Recombinant

Format

Purity: >95%
Conjugate/Label: Unconjugated
Endotoxin Level: < 0.2 EU/µg
Format: Lyophilized
Formulation: Lyophilized from a 0.2 um filtered solution in 20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 2 mM CaCl2.
Purity Measurement Method: SDS-PAGE (Coomassie Stain)