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BOP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BOP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all BOP1 antibodies →
$504.00each
Catalog Number: LS-G113391-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BOP1 (NM_015201), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BOP1 | Block of proliferation 1 | KIAA0124
Origin Species
Human
NCBI GenBank
AX077673
SwissProt
Q14137

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGSRGAGRT AAPSVRPEKR RSEPELEPEP EPEPPLLCTS PLSHSTGSDS GVSDSEESVF
61SGLEDSGSDS SEDDDEGDEE GEDGALDDEG HSGIKKTTEE QVQASTPCPR TEMASARIGD
121EYAEDSSDEE DIRNTVGNVP LEWYDDFPHV GYDLDGRRIY KPLRTRDELD QFLDKMDDPD
181YWRTVQDPMT GRDLRLTDEQ VALVRRLQSG QFGDVGFNPY EPAVDFFSGD VMIHPVTNRP
241ADKRSFIPSL VEKEKVSRMV HAIKMGWIQP RRPRDPTPSF YDLWAQEDPN AVLGRHKMHV
301PAPKLALPGH AESYNPPPEY LLSEEERLAW EQQEPGERKL SFLPRKFPSL RAVPAYGRFI
361QERFERCLDL YLCPRQRKMR VNVDPEDLIP KLPRPRDLQP FPTCQALVYR GHSDLVRCLS
421VSPGGQWLVS GSDDGSLRLW EVATARCVRT VPVGGVVKSV AWNPSPAVCL VAAAVEDSVL
481LLNPALGDRL VAGSTDQLLS AFVPPEEPPL QPARWLEASE EERQVGLRLR ICHGKPVTQV
541TWHGRGDYLA VVLATQGHTQ VLIHQLSRRR SQSPFRRSHG QVQRVAFHPA RPFLLVASQR
601SVRLYHLLRQ ELTKKLMPNC KWVSSLAVHP AGDNVICGSY DSKLVWFDLD LSTKPYRMLR
661HHKKALRAVA FHPRYPLFAS GSDDGSVIVC HGMVYNDLLQ NPLLVPVKVL KGHVLTRDLG
721VLDVIFHPTQ PWVFSSGADG TVRLFT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

746 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.