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BOC Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BOC Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all BOC antibodies →
$725.00each
Catalog Number: LS-G108355-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BOC (NM_033254), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Brother of CDO | BOC | Boc homolog (mouse) | Protein BOC | CDON2
Origin Species
Human
NCBI GenBank
N31808
SwissProt
Q9BWV1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
120.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MLRGTMTAWR GMRPEVTLAC LLLATAGCFA DLNEVPQVTV QPASTVQKPG GTVILGCVVE
61PPRMNVTWRL NGKELNGSDD ALGVLITHGT LVITALNNHT VGRYQCVARM PAGAVASVPA
121TVTLANLQDF KLDVQHVIEV DEGNTAVIAC HLPESHPKAQ VRYSVKQEWL EASRGNYLIM
181PSGNLQIVNA SQEDEGMYKC AAYNPVTQEV KTSGSSDRLR VRRSTAEAAR IIYPPEAQTI
241IVTKGQSLIL ECVASGIPPP RVTWAKDGSS VTGYNKTRFL LSNLLIDTTS EEDSGTYRCM
301ADNGVGQPGA AVILYNVQVF EPPEVTMELS QLVIPWGQSA KLTCEVRGNP PPSVLWLRNA
361VPLISSQRLR LSRRALRVLS MGPEDEGVYQ CMAENEVGSA HAVVQLRTSR PSITPRLWQD
421AELATGTPPV SPSKLGNPEQ MLRGQPALPR PPTSVGPASP QCPGEKGQGA PAEAPIILSS
481PRTSKTDSYE LVWRPRHEGS GRAPILYYVV KHRKVTNSSD DWTISGIPAN QHRLTLTRLD
541PGSLYEVEMA AYNCAGEGQT AMVTFRTGRR PKPEIMASKE QQIQRDDPGA SPQSSSQPDH
601GRLSPPEAPD RPTISTASET SVYVTWIPRG NGGFPIQSFR VEYKKLKKVG DWILATSAIP
661PSRLSVEITG LEKGTSYKFR VRALNMLGES EPSAPSRPYV VSGYSGRVYE RPVAGPYITF
721TDAVNETTIM LKWMYIPASN NNTPIHGFYI YYRPTDSDND SDYKKDMVEG DKYWHSISHL
781QPETSYDIKM QCFNEGGESE FSNVMICETK ARKSSGQPGR LPPPTLAPPQ PPLPETIERP
841VGTGAMVARS SDLPYLIVGV VLGSIVLIIV TFIPFCLWRA WSKQKHTTDL GFPRSALPPS
901CPYTMVPLGG LPGHQASGQP YLSGISGRAC ANGIHMNRGC PSAAVGYPGM KPQQHCPGEL
961QQQSDTSSLL RQTHLGNGYD PQSHQITRGP KSSPDEGSFL YTLPDDSTHQ LLQPHHDCCQ
1021RQEQPAAVGQ SGVRRAPDSP VLEAVWDPPF HSGPPCCLGL VPVEEVDSPD SCQVSGGDWC
1081PQHPVGAYVG QEPGMQLSPG PLVRVSFETP PLTI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1114 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.