Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BMP2K / BIKE Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103538-100
Catalog Number: LS-G103538
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BMP2K (NM_198892), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BMP2 inducible kinase | BIKE | BMP-2-inducible protein kinase | BMP-2 inducible kinase | BMP2K | HRIHFB2017 | Bike kinase
- Origin Species
- Human
- NCBI GenBank
- NM_017593
- SwissProt
- Q9NSY1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 129 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKKFSRMPKS EGGSGGGAAG GGAGGAGAGA GCGSGGSSVG VRVFAVGRHQ VTLEESLAEG61GFSTVFLVRT HGGIRCALKR MYVNNMPDLN VCKREITIMK ELSGHKNIVG YLDCAVNSIS121DNVWEVLILM EYCRAGQVVN QMNKKLQTGF TEPEVLQIFC DTCEAVARLH QCKTPIIHRD181LKVENILLND GGNYVLCDFG SATNKFLNPQ KDGVNVVEEE IKKYTTLSYR APEMINLYGG241KPITTKADIW ALGCLLYKLC FFTLPFGESQ VAICDGNFTI PDNSRYSRNI HCLIRFMLEP301DPEHRPDIFQ VSYFAFKFAK KDCPVSNINN SSIPSALPEP MTASEAAARK SQIKARITDT361IGPTETSIAP RQRPKANSAT TATPSVLTIQ SSATPVKVLA PGEFGNHRPK GALRPGNGPE421ILLGQGPPQQ PPQQHRVLQQ LQQGDWRLQQ LHLQHRHPHQ QQQQQQQQQQ QQQQQQQQQQ481QQQQQQHHHH HHHHLLQDAY MQQYQHATQQ QQMLQQQFLM HSVYQPQPSA SQYPTMMPQY541QQAFFQQQML AQHQPSQQQA SPEYLTSPQE FSPALVSYTS SLPAQVGTIM DSSYSANRSV601ADKEAIANFT NQKNISNPPD MSGWNPFGED NFSKLTEEEL LDREFDLLRS NRLEERASSD661KNVDSLSAPH NHPPEDPFGS VPFISHSGSP EKKAEHSSIN QENGTANPIK NGKTSPASKD721QRTGKKTSVQ GQVQKGNDES ESDFESDPPS PKSSEEEEQD DEEVLQGEQG DFNDDDTEPE781NLGHRPLLMD SEDEEEEEKH SSDSDYEQAK AKYSDMSSVY RDRSGSGPTQ DLNTILLTSA841QLSSDVAVET PKQEFDVFGA VPFFAVRAQQ PQQEKNEKNL PQHRFPAAGL EQEEFDVFTK901APFSKKVNVQ ECHAVGPEAH TIPGYPKSVD VFGSTPFQPF LTSTSKSESN EDLFGLVPFD961EITGSQQQKV KQRSLQKLSS RQRRTKQDMS KSNGKRHHGT PTSTKKTLKP TYRTPERARR1021HKKVGRRDSQ SSNEFLTISD SKENISVALT DGKDRGNVLQ PEESLLDPFG AKPFHSPDLS1081WHPPHQGLSD IRADHNTVLP GRPRQNSLHG SFHSADVLKM DDFGAVPFTE LVVQSITPHQ1141SQQSQPVELD PFGAAPFPSK QTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1161 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.