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BIVM Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BIVM Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112280-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BIVM (NM_017693), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BIVM
Origin Species
Human
NCBI GenBank
NM_017693
SwissProt
Q86UB2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPNVAETERS NDSGNGEHKS ERKSPEENLQ GAVKSFCTSA SGAPLGPKGD GHYPWSCPVT
61HTREKIYAIC SDYAFLNQAT SIYKTPNPSR SPCLPDSTSL SAGNNSSRYI GIPTSTSEII
121YNEENSLENL SNSLGKLPLA WEIDKSEFDG VTTNSKHKSG NAKKQVSKRK TSDKKGRYQK
181ECPQHSPLED IKQRKVLDLR RWYCISRPQY KTSCGISSLI SCWNFLYSTM GAGNLPPITQ
241EEALHILGFQ PPFEDIRFGP FTGNTTLMRW FRQINDHFHV KGCSYVLYKP HGKNKTAGET
301ASGALSKLTR GLKDESLAYI YHCQNHYFCP IGFEATPVKA NKAFSRGPLS PQEVEYWILI
361GESSRKHPAI HCKKWADIVT DLNTQNPEYL DIRHLERGLQ YRKTKKVGGN LHCIIAFQRL
421NWQRFGLWNF PFGTIRQESQ PPTHAQGIAK SESEDNISKK QHGRLGRSFS ASFHQDSAWK
481KMSSIHERRN SGYQGYSDYD GND
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

503 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.