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BIN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BIN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all BIN2 antibodies →
$504.00each
Catalog Number: LS-G112740-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BIN2 (NM_016293), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BRAP1 | BIN2 | BRAP-1 | Bridging integrator 2 | Bridging integrator-2
Origin Species
Human
NCBI GenBank
NM_016293
SwissProt
Q9UBW5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEGKAGGAA GLFAKQVQKK FSRAQEKVLQ KLGKAVETKD ERFEQSASNF YQQQAEGHKL
61YKDLKNFLSA VKVMHESSKR VSETLQEIYS SEWDGHEELK AIVWNNDLLW EDYEEKLADQ
121AVRTMEIYVA QFSEIKERIA KRGRKLVDYD SARHHLEAVQ NAKKKDEAKT AKAEEEFNKA
181QTVFEDLNQE LLEELPILYN SRIGCYVTIF QNISNLRDVF YREMSKLNHN LYEVMSKLEK
241QHSNKVFVVK GLSSSSRRSL VISPPVRTAT VSSPLTSPTS PSTLSLKSES ESVSATEDLA
301PDAAQGEDNS EIKELLEEEE IEKEGSEASS SEEDEPLPAC NGPAQAQPSP TTERAKSQEE
361VLPSSTTPSP GGALSPSGQP SSSATEVVLR TRTASEGSEQ PKKRASIQRT SAPPSRPPPP
421RATASPRPSS GNIPSSPTAS GGGSPTSPRA SLGTGTASPR TSLEVSPNPE PPEKPVRTPE
481AKENENIHNQ NPEELCTSPT LMTSQVASEP GEAKKMEDKE KDNKLISADS SEGQDQLQVS
541MVPENNNLTA PEPQEEVSTS ENPQL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

565 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.