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BICD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BICD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G118437-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BICD1 (NM_001714), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Bic-D 1 | BICD | BICD1 | Protein bicaudal D homolog 1
Origin Species
Human
NCBI GenBank
NM_001714
SwissProt
Q96G01

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
110.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAEEVLQTV DHYKTEIERL TKELTETTHE KIQAAEYGLV VLEEKLTLKQ QYDELEAEYD
61SLKQELEQLK EAFGQSFSIH RKVAEDGETR EETLLQESAS KEAYYLGKIL EMQNELKQSR
121AVVTNVQAEN ERLTAVVQDL KENNEMVELQ RIRMKDEIRE YKFREARLLQ DYTELEEENI
181TLQKLVSTLK QNQVEYEGLK HEIKRFEEET VLLNSQLEDA IRLKEIAEHQ LEEALETLKN
241EREQKNNLRK ELSQYISLND NHISISVDGL KFAEDGSEPN NDDKMNGHIH GPLVKLNGDY
301RTPTLRKGES LNPVSDLFSE LNISEIQKLK QQLMQVEREK AILLANLQES QTQLEHTKGA
361LTEQHERVHR LTEHVNAMRG LQSSKELKAE LDGEKGRDSG EEAHDYEVDI NGLEILECKY
421RVAVTEVIDL KAEIKALKEK YNKSVENYTD EKAKYESKIQ MYDEQVTSLE KTTKESGEKM
481AHMEKELQKM TSIANENHST LNTAQDELVT FSEELAQLYH HVCLCNNETP NRVMLDYYRQ
541SRVTRSGSLK GPDDPRGLLS PRLARRGVSS PVETRTSSEP VAKESTEASK EPSPTKTPTI
601SPVITAPPSS PVLDTSDIRK EPMNIYNLNA IIRDQIKHLQ KAVDRSLQLS RQRAAARELA
661PMIDKDKEAL MEEILKLKSL LSTKREQIAT LRAVLKANKQ TAEVALANLK NKYENEKAMV
721TETMTKLRNE LKALKEDAAT FSSLRAMFAT RCDEYVTQLD EMQRQLAAAE DEKKTLNTLL
781RMAIQQKLAL TQRLEDLEFD HEQSRRSKGK LGKSKIGSPK VSGEASVTVP TIDTYLLHSQ
841GPQTPNIRVS SGTQRKRQFS PSLCDQSRPR TSGASYLQNL LRVPPDPTST ESFLLKGPPS
901MSEFIQGHRL SKEKRLTVAP PDCQQPAASV PPQCSQLAGR QDCPTVSPDT ALPEEQPHSS
961SQCAPLHCLS KPPHP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

975 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.