Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BIA2 / TRIM58 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101318-100
Catalog Number: LS-G101318
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIM58 (NM_015431), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BIA2 | TRIM58 | Tripartite motif-containing 58 | Tripartite motif containing 58 | Protein BIA2
- Origin Species
- Human
- NCBI GenBank
- AL080170
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASAPPGERL REDARCPVCL DFLQEPVSVD CGHSFCLRCI SEFCEKSDGA QGGVYACPQC61RGPFRPSGFR PNRQLAGLVE SVRRLGLGAG PGARRCARHG EDLSRFCEED EAALCWVCDA121GPEHRTHRTA PLQEAAGSYQ VKLQMALELM RKELEDALTQ EANVGKKTVI WKEKVEMQRQ181RFRLEFEKHR GFLAQEEQRQ LRRLEAEERA TLQRLRESKS RLVQQSKALK ELADELQERC241QRPALGLLEG VRGVLSRSKA VTRLEAENIP MELKTACCIP GRRELLRKFQ VDVKLDPATA301HPSLLLTADL RSVQDGEPWR DVPNNPERFD TWPCILGLQS FSSGRHYWEV LVGEGAEWGL361GVCQDTLPRK GETTPSPENG VWALWLLKGN EYMVLASPSV PLLQLESPRC IGIFLDYEAG421EISFYNVTDG SYIYTFNQLF SGLLRPYFFI CDATPLILPP TTIAGSGNWA SRDHLDPASD481VRDDHLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
486 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.