Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BEST2 / Bestrophin-2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101492-100
Catalog Number: LS-G101492
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Bestrophin 2(BEST2) (NM_017682), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BEST2 | Bestrophin-2 | Bestrophin 2 | Hbest2 | VMD2-like gene 1 | VMD2L1
- Origin Species
- Human
- NCBI GenBank
- NM_017682
- SwissProt
- Q8NFU1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTVTYTARVA NARFGGFSQL LLLWRGSIYK LLWRELLCFL GFYMALSAAY RFVLTEGQKR61YFEKLVIYCD QYASLIPVSF VLGFYVTLVV NRWWSQYLCM PLPDALMCVV AGTVHGRDDR121GRLYRRTLMR YAGLSAVLIL RSVSTAVFKR FPTIDHVVEA GFMTREERKK FENLNSSYNK181YWVPCVWFSN LAAQARREGR IRDNSALKLL LEELNVFRGK CGMLFHYDWI SVPLVYTQVV241TIALYSYFLA CLIGRQFLDP AQGYKDHDLD LCVPIFTLLQ FFFYAGWLKV AEQLINPFGE301DDDDFETNFL IDRNFQVSML AVDEMYDDLA VLEKDLYWDA AEARAPYTAA TVFQLRQPSF361QGSTFDITLA KEDMQFQRLD GLDGPMGEAP GDFLQRLLPA GAGMVAGGPL GRRLSFLLRK421NSCVSEASTG ASCSCAVVPE GAAPECSCGD PLLDPGLPEP EAPPPAGPEP LTLIPGPVEP481FSIVTMPGPR GPAPPWLPSP IGEEEENLATRTRPLEQKLISEEDLAANDILDYKDDDDKV
509 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.