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BEND4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BEND4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all BEND4 antibodies →
$725.00each
Catalog Number: LS-G102801-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BEND4 (NM_207406), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BEN domain containing 4 | BEND4 | CCDC4
Origin Species
Human
SwissProt
Q6ZU67

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEEMQPAEE GPSVPKIYKQ RSPYSVLKTF PSKRPALAKR YERPTLVELP HVRAPPPPPP
61PFAPHAAVSI SSSEPPPQQF QAQSSYPPGP GRAAAAASSS SPSCTPATSQ GHLRTPAQPP
121PASPAASSSS SFAAVVRYGP GAAAAAGTGG TGSDSASLEL SAESRMILDA FAQQCSRVLS
181LLNCGGKLLD SNHSQSMISC VKQEGSSYNE RQEHCHIGKG VHSQTSDNVD IEMQYMQRKQ
241QTSAFLRVFT DSLQNYLLSG SFPTPNPSSA SEYGHLADVD PLSTSPVHTL GGWTSPATSE
301SHGHPSSSTL PEEEEEEDEE GYCPRCQELE QEVISLQQEN EELRRKLESI PVPCQTVLDY
361LKMVLQHHNQ LLIPQPADQP TEGSKQLLNN YPVYITSKQW DEAVNSSKKD GRRLLRYLIR
421FVFTTDELKY SCGLGKRKRS VQSGETGPER RPLDPVKVTC LREFIRMHCT SNPDWWMPSE
481EQINKVFSDA VGHARQGRAV GTFLHNGGSF YEGIDHQASQ DEVFNKSSQD GSGD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

534 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.