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BEND3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BEND3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119326-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BEND3 (NM_001080450), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BEN domain containing 3 | KIAA1553 | RP11-59I9.2 | BEND3
Origin Species
Human
NCBI GenBank
AB046773
SwissProt
Q5T5X7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSTEFTEDV EEVLKSITVK VETEAEDAAL DCSVNSRTSE KHSVDSVLTA LQDSSKRKQL
61VSDGLLDSVP GVKRRRLIPE ALLAGMRNRE NSSPCQGNGE QAGRGRSLGN VWPGEEEPCN
121DATTPSYKKP LYGISHKIME KKNPPSGDLL NVYELFEKAN ASNSPSSLRL LNEPQKRDCG
181STGAGTDNDP NIYFLIQKMF YMLNTLTSNM SQLHSKVDLL SLEVSRIKKQ VSPTEMVAKF
241QPPPEYQLTA AELKQIVDQS LSGGDLACRL LVQLFPELFS DVDFSRGCSA CGFAAKRKLE
301SLHLQLIRNY VEVYYPSVKD TAVWQAECLP QLNDFFSRFW AQREMEDSQP SGQVASFFEA
361EQVDPGHFLD NKDQEEALSL DRSSTIASDH VVDTQDLTEF LDEASSPGEF AVFLLHRLFP
421ELFDHRKLGE QYSCYGDGGK QELDPQRLQI IRNYTEIYFP DMQEEEAWLQ QCAQRINDEL
481EGLGLDAGSE GDPPRDDCYD SSSLPDDISV VKVEDSFEGE RPGRRSKKIW LVPIDFDKLE
541IPQPDFEVPG ADCLLSKEQL RSIYESSLSI GNFASRLLVH LFPELFTHEN LRKQYNCSGS
601LGKKQLDPSR IKLIRHYVQL LYPRAKNDRV WTLEFVGKLD ERCRRRDTEQ RRSYQQQRKV
661HVPGPECRDL TSYAINPERF REEFEGPPLP PERSSKDFCK IPLDELVVPS PDFPVPSPYL
721LSDKEVREIV QQSLSVGNFA ARLLVRLFPE LFTAENLRLQ YNHSGACNKK QLDPTRLRLI
781RHYVEAVYPV EKMEEVWHYE CIPSIDERCR RPNRKKCDIL KKAKKVEK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

828 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.