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BEND2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BEND2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all BEND2 antibodies →
$504.00each
Catalog Number: LS-G105793-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CXorf20(BEND2) (NM_153346), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BEND2 | BEN domain containing 2 | CXorf20
Origin Species
Human
NCBI GenBank
W06387

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSERTQEQDF VIITVDDSDD NNDCSIEMVE VSETADNSTN DIADDSTYVT ADNPTDDTAT
61QPNFPGGNDG HHRPLQMSYG SGSVTQAGVQ WHDHSSLQPQ PLGLKQFFHL SLPSSWDDRR
121TPPCPVAHGD QIVSQINHPV HLRRYSYNSE EVDFPKRGRF YTPEVQSSIS PPAERQETHA
181WASPAVTSLE SAACHELQEA DLSESLSYPR IVSSSSLQQY VAQGGSFPCF GMPWNFISGG
241AESTNAVISF ANATTAVPMA VLSRRESSLA NNPGVVNYSA LPENENVGPG RALSSFCFHP
301NLEMPERPAN SSKNSTETAN YPTLMGNYNG QNTASLSVFI PPYFAEKIIL TEMPGTTETN
361VENNSQTVYY PALSGNTSAP YPASSYLPIT SNFESGPQMS YGTMSYSTEM KNNCDQDDAS
421ASACLTPDFA LLPLNILVKV DTNTENSVNT MNRSTLLDSD SGQDSSSSSV CIPPKYGYLG
481DPKRNVRVLK IHLLAVQNMA KPKQAACYLV RILFSKEILI SSSVDIHLKD SQSLDPNKMA
541ALREYLATTF PTCDLHEHGK DWQDCISGIN SMIYCLCSEG KSTPKTVRKN KKRTNRVASA
601SADRNDQRGR DGGEGCSWMF QPMNNSKMRV KRNLQPNSNA IPEGMREPST DNPEEPGEAW
661SYFGRPWRNI RMPCSVLTLA KTKSCASLSA RYLIQKLFTK DVLVQSNVYG NLKHGLCALD
721PNKISALREF LQENYPICDL SENGRDWKSC VTSINSGIRS LRHDVRRAEA RSQSLPAVTP
781PELEQESKPG DPDATDPST
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

799 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.