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BEGAIN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BEGAIN Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110945-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BEGAIN (NM_020836), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BEGAIN | KIAA1446
Origin Species
Human
NCBI GenBank
NM_020836
SwissProt
Q9BUH8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKLSALQEQ KGELRKRLSY TTHKLEKLET EFDSTRHYLE IELRRAQEEL EKVTEKLRRI
61QSNYMALQRI NQELEDKLYR MGQHYEEEKR ALSHEIVALN SHLLEAKVTI DKLSEDNELY
121RKDCNLAAQL LQCSQTYGRV HKVSELPSDF QERVSLHMEK HGCSLPSPLC HPAYADSVPT
181CVIAKVLEKP DPASLSSRLS DASARDLAFC DGVEKPGPRP PYKGDIYCSD TALYCPEERR
241RDRRPSVDAP VTDVGFLRAQ NSTDSAAEEE EEAEAAAFPA GFQHEAFPSY AGSLPTSSSY
301SSFSATSEEK EHAQASTLTA SQQAIYLNSR DELFDRKPPA TTYEGSPRFA KATAAVAAPL
361EAEVAPGFGR TMSPYPAETF RFPASPGPQQ ALMPPNLWSL RAKPGTARLP GEDMRGQWRP
421LSVEDIGAYS YPVSAAGRAS PCSFSERYYG GAGGSPGKKA DGRASPLYAS YKADSFSEGD
481DLSQGHLAEP CFLRAGGDLS LSPGRSADPL PGYAPSEGGD GDRLGVQLCG TASSPEPEQG
541SRDSLEPSSM EASPEMHPAA RLSPQQAFPR TGGSGLSRKD SLTKAQLYGT LLNTRTRPLE
601QKLISEEDLA ANDILDYKDD DDKV

624 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.