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BCOR Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BCOR Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112245-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BCOR (NM_017745), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BCOR | ANOP2 | BCL6 co-repressor | BCL-6 corepressor | BCL6 corepressor | KIAA1575 | MAA2 | BCL-6 interacting corepressor | MCOPS2
Origin Species
Human
NCBI GenBank
NM_017745
SwissProt
Q6W2J9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
188 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLSATPLYGN VHSWMNSERV RMCGASEDRK ILVNDGDASK ARLELREENP LNHNVVDAST
61AHRIDGLAAL SMDRTGLIRE GLRVPGNIVY SSLCGLGSEK GREAATSTLG GLGFSSERNP
121EMQFKPNTPE TVEASAVSGK PPNGFSAIYK TPPGIQKSAV ATAEALGLDR PASDKQSPLN
181INGASYLRLP WVNPYMEGAT PAIYPFLDSP NKYSLNMYKA LLPQQSYSLA QPLYSPVCTN
241GERFLYLPPP HYVGPHIPSS LASPMRLSTP SASPAIPPLV HCADKSLPWK MGVSPGNPVD
301SHAYPHIQNS KQPRVPSAKA VTSGLPGDTA LLLPPSPRPS PRVHLPTQPA ADTYSEFHKH
361YARISTSPSV ALSKPYMTVS SEFPAARLSN GKYPKAPEGG EGAQPVPGHA RKTAVQDRKD
421GSSPPLLEKQ TVTKDVTDKP LDLSSKVVDV DASKADHMKK MAPTVLVHSR AGSGLVLSGS
481EIPKETLSPP GNGCAIYRSE IISTAPSSWV VPGPSPNEEN NGKSMSLKNK ALDWAIPQQR
541SSSCPRMGGT DAVITNVSGS VSSAGRPASA SPAPNANADG TKTSRSSVET TPSVIQHVGQ
601PPATPAKHSS STSSKGAKAS NPEPSFKANE NGLPPSSIFL SPNEAFRSPP IPYPRSYLPY
661PAPEGIAVSP LSLHGKGPVY PHPVLLPNGS LFPGHLAPKP GLPYGLPTGR PEFVTYQDAL
721GLGMVHPMLI PHTPIEITKE EKPERRSRSH ERARYEDPTL RNRFSEILET SSTKLHPDVP
781TDKNLKPNPN WNQGKTVVKS DKLVYVDLLR EEPDAKTDTN VSKPSFAAES VGQSAEPPKP
841SVEPALQQHR DFIALREELG RISDFHETYT FKQPVFTVSK DSVLAGTNKE NLGLPVSTPF
901LEPPLGSDGP AVTFGKTQED PKPFCVGSAP PSVDVTPTYT KDGADEAESN DGKVLKPKPS
961KLAKRIANSA GYVGDRFKCV TTELYADSSQ LSREQRALQM EGLQEDSILC LPAAYCERAM
1021MRFSELEMKE REGGHPATKD SEMCKFSPAD WERLKGNQDK KPKSVTLEEA IAEQNESERC
1081EYSVGNKHRD PFEAPEDKDL PVEKYFVERQ PVSEPPADQV ASDMPHSPTL RVDRKRKVSG
1141DSSHTETTAE EVPEDPLLKA KRRRVSKGLH PKKQRHLLHL RERWEQQVSA ADGKPGRQSR
1201KEVTQATQPE AIPQGTNITE EKPGRKRAEA KGNRSWSEES LKPSDNEQGL PVFSGSPPMK
1261SLSSTSAGGK KQAQPSCAPA SRPPAKQQKI KENQKTDVLC ADEEEDCQAA SLLQKYTDNS
1321EKPSGKRLCK TKHLIPQESR RGLPLTGEYY VENADGKVTV RRFRKRPEPS SDYDLSPAKQ
1381EPKPFDRLQQ LLPASQSTQL PCSSSPQETT QSRPMPPEAR RLIVNKNAGE TLLQRAARLG
1441YEEVVLYCLE NKICDVNHRD NAGYCALHEA CARGWLNIVR HLLEYGADVN CSAQDGTRPL
1501HDAVENDHLE IVRLLLSYGA DPTLATYSGR TIMKMTHSEL MEKFLTDYLN DLQGRNDDDA
1561SGTWDFYGSS VCEPDDESGY DVLANPPGPE DQDDDDDAYS DVFEFEFSET PLLPCYNIQV
1621SVAQGPRNWL LLSDVLKKLK MSSRIFRCNF PNVEIVTIAE AEFYRQVSAS LLFSCSKDLE
1681AFNPESKELL DLVEFTNEIQ TLLGSSVEWL HPSDLASDNY W
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1721 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.