Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BCO2 / BCDO2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120495-100
Catalog Number: LS-G120495
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BCO2 (NM_001037290), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- B | b-carotene-9 | 10-dioxygenase | B-DIOX-II | BCDO2 | Beta-carotene 9 | 10 oxygenase | BCO2 | Beta-carotene dioxygenase 2 | Beta-carotene oxygenase 2
- Origin Species
- Human
- NCBI GenBank
- NM_031938
- SwissProt
- Q9BYV7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGNTPQKKAV FGQCRGLPCV APLLTTVEEA PRGISARVWG HFPKWLNGSL LRIGPGKFEF61GKDKYNHWFD GMALLHQFRM AKGTVTYRSK FLQSDTYKAN SAKNRIVISE FGTLALPDPC121KNVFERFMSR FELPGKAAAM TDNTNVNYVR YKGDYYLCTE TNFMNKVDIE TLEKTEKVDW181SKFIAVNGAT AHPHYDLDGT AYNMGNSFGP YGFSYKVIRV PPEKVDLGET IHGVQVICSI241ASTEKGKPSY YHSFGMTRNY IIFIEQPLKM NLWKIATSKI RGKAFSDGIS WEPQCNTRFH301VVEKRTGQLL PGRYYSKPFV TFHQINAFED QGCVIIDLCC QDNGRTLEVY QLQNLRKAGE361GLDQVHNSAA KSFPRRFVLP LNVSLNAPEG DNLSPLSYTS ASAVKQADGT IWCSHENLHQ421EDLEKEGGIE FPQIYYDRFS GKKYHFFYGC GFRHLVGDSL IKVDVVNKTL KVWREDGFYP481SEPVFVPAPG TNEEDGGVIL SVVITPNQNE SNFILVLDAK NFEELGRAEV PVQMPYGFHG541TFIPITRTRPLEQKLISEEDLAANDILDYKDDDDKV
545 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.