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BCL6B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BCL6B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G104313-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BCL6B (NM_181844), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BCL6B | BAZF | ZBTB28 | ZNF62 | Zinc finger protein 62
Origin Species
Human
SwissProt
Q8N143

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSPAAPEGA LGYVREFTRH SSDVLGNLNE LRLRGILTDV TLLVGGQPLR AHKAVLIACS
61GFFYSIFRGR AGVGVDVLSL PGGPEARGFA PLLDFMYTSR LRLSPATAPA VLAAATYLQM
121EHVVQACHRF IQASYEPLGI SLRPLEAEPP TPPTAPPPGS PRRSEGHPDP PTESRSCSQG
181PPSPASPDPK ACNWKKYKYI VLNSQASQAG SLVGERSSGQ PCPQARLPSG DEASSSSSSS
241SSSSSEEGPI PGPQSRLSPT AATVQFKCGA PASTPYLLTS QAQDTSGSPS ERARPLPGSE
301FFSCQNSEAV AGCSSGLDSL VPGDEDKPYK CQLCRSSFRY KGNLASHRTV HTGEKPYHCS
361ICGARFNRPA NLKTHSRIHS GEKPYKCETC GSRFVQVAHL RAHVLIHTGE KPYPCPTCGT
421RFRHLQTLKS HVRIHTGEKP YHCDPCGLHF RHKSQLRLHL RQKHGAATNT KVHYHILGGP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

480 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.