Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BCL3 / BCL-3 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116131-100
Catalog Number: LS-G116131
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BCL3 (NM_005178), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- B-cell CLL/lymphoma 3 | B-cell leukemia/lymphoma 3 | B-cell lymphoma 3 protein | BCL-3 | BCL3 | BCL4 | D19S37 | Proto-oncogene BCL3
- Origin Species
- Human
- NCBI GenBank
- NM_005178
- SwissProt
- P20749
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDEGPVDLRT RPKAAGLPGA ALPLRKRPLR APSPEPAAPR GAAGLVVPLD PLRGGCDLPA61VPGPPHGLAR PEALYYPGAL LPLYPTRAMG SPFPLVNLPT PLYPMMCPME HPLSADIAMA121TRADEDGDTP LHIAVVQGNL PAVHRLVNLF QQGGRELDIY NNLRQTPLHL AVITTLPSVV181RLLVTAGASP MALDRHGQTA AHLACEHRSP TCLRALLDSA APGTLDLEAR NYDGLTALHV241AVNTECQETV QLLLERGADI DAVDIKSGRS PLIHAVENNS LSMVQLLLQH GANVNAQMYS301GSSALHSASG RGLLPLVRTL VRSGADSSLK NCHNDTPLMV ARSRRVIDIL RGKATRPAST361SQPDPSPDRS ANTSPESSSR LSSNGLLSAS PSSSPSQSPP RDPPGFPMAP PNFFLPSPSP421PAFLPFAGVL RGPGRPVPPS PAPGGSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
446 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.