Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BCL2L14 / BCL-G Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107241-100
Catalog Number: LS-G107241
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Bcl G(BCL2L14) (NM_138722), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Bcl2-L-14 | BCLG | BCL-G | BCL2L14 | Apoptosis regulator Bcl-G
- Origin Species
- Human
- NCBI GenBank
- NM_030766
- SwissProt
- Q9BZR8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MCSTSGCDLE EIPLDDDDLN TIEFKILAYY TRHHVFKSTP ALFSPKLLRT RSLSQRGLGN61CSANESWTEV SWPCRNSQSS EKAINLGKKK SSWKAFFGVV EKEDSQSTPA KVSAQGQRTL121EYQDSHSQQW SRCLSNVEQC LEHEAVDPKV ISIANRVAEI VYSWPPPQAT QAGGFKSKEI181FVTEGLSFQL QGHVPVASSS KKDEEEQILA KIVELLKYSG DQLERKLKKD KALMGHFQDG241LSYSVFKTIT DQVLMGVDPR GESEVKAQGF KAALVIDVTA KLTAIDNHPM NRVLGFGTKY301LKENFSPWIQ QHGGWEKILG ISHEEVDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
327 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.