Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BCL2L13 / Bcl Rambo Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113265-100
Catalog Number: LS-G113265
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Bcl rambo(BCL2L13) (NM_015367), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BCL-RAMBO | Bcl-2-like protein 13 | BCL2L13 | Protein Mil1 | Bcl2-L-13 | MIL1
- Origin Species
- Human
- NCBI GenBank
- NM_015367
- SwissProt
- Q9BXK5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASSSTVPLG FHYETKYVVL SYLGLLSQEK LQEQHLSSPQ GVQLDIASQS LDQEILLKVK61TEIEEELKSL DKEISEAFTS TGFDRHTSPV FSPANPESSM EDCLAHLGEK VSQELKEPLH121KALQMLLSQP VTYQAFRECT LETTVHASGW NKILVPLVLL RQMLLELTRR GQEPLSALLQ181FGVTYLEDYS AEYIIQQGGW GTVFSLESEE EEYPGITAED SNDIYILPSD NSGQVSPPES241PTVTTSWQSE SLPVSLSASQ SWHTESLPVS LGPESWQQIA MDPEEVKSLD SNGAGEKSEN301NSSNSDIVHV EKEEVPEGME EAAVASVVLP ARELQEALPE APAPLLPHIT ATSLLGTRES361DTEVITVEKS SPATSLFVEL DEEEVKAATT EPTEVEEVVP ALEPTETLLS EKEINAREES421LVEELSPASE KKPVPPSEGK SRLSPAGEMK PMPLSEGKSI LLFGGAAAVA ILAVAIGVAL481ALRKKTRTRP LEQKLISEED LAANDILDYK DDDDKV
516 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.